Fig. 1.

Overexpression of PLP1 promotes PEDV replication and inhibits IFN-β promoter activation induced by TNF-α.

(A) Vero E6 cells were transfected with pCMV-PLP1-HA or empty pCMV-HA vector. At 24 hpt, cells were infected with PEDV at MOI of 0.1. At 24 hpi, viral RNA levels were determined. (B) HEK293 T cells were seeded in a 24-well plate and cotransfected with IFN-Luc, TK-Luc, and pCMV-PLP1-HA or empty pCMV-HA vector. At 24 hpt, the cells were stimulated with 10 ng/mL TNF-α for 6 h and cell lysates were prepared for luciferase analysis. (C) HEK293 T were stimulated with TNF-α (10 ng/mL), then transfected with pCMV-PLP1-HA or empty pCMV-HA vector. Levels of IFN mRNA were determined by quantitative RT-PCR at 12, 24, 36 and 48 hpi.