Figure 4.

Different process schemes which were evaluated for bioethanol production (BP) of GL (EH: enzymatic hydrolysis). (a) the whole slurry of all thermo-chemical pretreatments was used for BP using Pichia stipitis in SSF (simultaneous saccharification and fermentation) mode. The whole slurry of pretreatment with H₂SO₄ and H₃PO₄, 10 g/100 g TS and HCl, 20 g/100 g TS was used for BP (b) using P. stipitis, Pachysolen tannophilus and Saccharomyces cerevisiae in SSF (c) or at separate hydrolysis and fermentation (SHF), (d) or separated and the liquid fraction was used for BP using P. stipitis/P. tannophilus, whereas the solid fraction was fermented by S. cerevisiae in SSF or SHF concept.