Table 1.

Absorbance at 336 nm (GSNO λ_max) of 1 mM GSNO in complete DMEM over 24 h. This data exhibits the decline of GSNO absorbance, particularly in the first 18 h of analysis. Proportionally, the concentration of GSNO and available NO decline over the 24 h period.

Time (h)	Absorbance at 336 nm (GSNO λmax)	Concentration of GSNO (mM)	Amount of Available NO (μmol)
0 h	0.895	0.971	0.433
6 h	0.598	0.649	0.290
12 h	0.304	0.330	0.147
18 h	0.130	0.141	0.0630
24 h	0.135	0.146	0.0650