Table 1. Genes of N. crassa modulated in response to low-Pi or high-Pi concentrations. Comparisons were made for the double mutant Δpac-3 strain considering the Δmus-52 strain as control (Martins et al., 2019) and for Δmus-52 strain considering the St.L.74-OR23-1VA strain as control (Martins et al., 2018). The selected genes are associated with morphology and development regulation, including the Gene Ontology descendants search of the nodes “cell wall,” “developmental process,” “cellular developmental process,” “regulation of biological process,” and “anatomical structure development”.

GO ID	Gene ID	Gene Product Name	Δpac-3 vs. Δmus-52		Δmus-52 vs. 74A
			low-Pi	high-Pi	low-Pi	high-Pi
GO:0006355	NCU04058‡ †	BZIP domain-containing protein		3.00	2.77
GO:0043666	NCU01504‡	Calcineurin binding protein		2.15
GO:0070884
GO:0006357	NCU08852‡	Poly(ADP-ribose) polymerase		2.18	2.66
GO:0030435	NCU08726‡ †	Fluffy		1.94
GO:0048315
GO:0006357
GO:0006355	NCU03650‡	DNA repair protein RAD16		1.55	1.77
Unannotated	NCU04197‡	CipC protein	6.62	8.44
GO:0006357	NCU00282‡ †	Zn(2)-C6 fungal-type domain-containing protein	3.89	4.34
GO:0046830	NCU07723‡	Norsolorinic acid reductase	3.08	2.26
GO:0006357	NCU04866‡ †	All development altered-6	2.00	2.15
GO:0033499	NCU04442‡	GAL10	1.73	1.77
GO:0000122	NCU08055‡ †	B-ZIP transcription factor IDI4	1.60	2.45
GO:0001080
GO:0045944
GO:1903833
GO:0007165	NCU06111‡	GTPase Ras2p	1.55	1.52
Unannotated	NCU09629‡	Hypothetical protein	-1.81	2.12		-3.14
Unannotated	NCU04605‡	Hypothetical protein	-2.61			-3.39
GO:0043934	NCU00586‡	Non-anchored cell wall protein-6	-2.48
GO:0006355	NCU00090‡ †	PH-response transcription factor pacC/RIM101	-1.80
GO:0045013	NCU03965‡	Catabolite repression protein creC	-1.78
GO:0072344	NCU03367‡	Hypothetical protein	-1.78
GO:0005619	NCU08791‡	Catalase-1	-1.64			-2.12
GO:0048315
GO:0061414	NCU02142‡ †	Zn(2)-C6 fungal-type domain-containing protein	-1.55		-4.62	-4.27
GO:0030968	NCU02235‡	Glycosyl hydrolase family 47-6		-6.03
GO:0006355	NCU01931‡	Hypothetical protein		-1.98
GO:0006357
GO:0030435	NCU00399‡	Cell wall protein PhiA		-1.79
GO:0007264	NCU02167‡	Krev-1-like		-1.57
Unannotated	NCU01064‡	Hypothetical protein	-8.84	-5.77
GO:1902600	NCU05046‡	E1-E2 ATPase-1	-6.77	-5.72
GO:0055114	NCU04452‡	Menadione induced gene-3	-5.18	-5.77
GO:1902600	NCU07966‡	Calcium-transporting ATPase 3	-4.00	-3.88
GO:0009277	NCU07253‡	1,3-beta-glucanosyltransferase gel1	-3.56	-3.26
GO:0016787	NCU07117‡	Ornithine-N5-oxygenase	-3.02	-2.28
GO:0061414	NCU00155‡ †	C6 transcription factor	-2.10	-1.86

Gene expression levels represented as log₂-fold change comparing test and control strains in each of the Pi conditions (Δpac-3 vs. Δmus-52 or Δmus-52 vs. N. crassa St.L.74-OR23-1VA, identified as 74A strain in the table). Only values of log2FoldChange> = 1.5 or <= −1.5 were represented.

^‡Genes presenting binding motif for PAC-3 in their promoter region.

^†Transcription factors