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. 2020 Jun 12;10(6):898. doi: 10.3390/biom10060898

Figure 3.

Figure 3

Microarray versus qPCR validation for three upregulated genes in OF-fed and control zebrafish. (A–C) The fold changes of oligo-fucoidan fed wild-type (WT+OF) zebrafish versus the no-treatment control (WT), where each dot represents the average of two fish from the same batch. (D–F) Quantitative polymerase chain reaction (qPCR) analyses for the same samples that were used in microarray, where each dot represents average of two fish from the same batch. (G–I) Quantitative polymerase chain reaction (qPCR) analyses for the all OF-fed wild-type fish (red) versus the untreated controls (black), where each dot represents one fish. Statistical analyses were performed with Student’s t-test (*0.01 < p ≤ 0.05; **0.001 < p ≤ 0.01; *** ≤ 0.001, ns: non-significance).