Figure 10.

Knockdown of Hnf4a and Asgpr1 in Clone 9 cells neutralized the OF-promoting effects in both Cyp3a4 activity and the cellular viability of normal liver cells. (A) Knockdown of Hnf4a abolished the fucoidan-mediated enhancement of Cyp3a4 activity. The blue color indicated sh-Hnf4a transfected cells, and the black color denoted the control transfected cells. The Cyp3a4 activities of the sh-Hnf4a knockdown or control cells were measured by CYP3A4 Activity Assay Kit. (B) The knockdown of Asgpr1 abolished the fucoidan-mediated enhancement of cellular viability in normal liver cells. The red color indicated first short hairpin RNA (shRNA) against Asgpr1-transfected cells, and the blue color denoted the second sh-RNA against Asgpr1-transfected cells, and the black color showed the control-transfected cells. The cellular viability was determined using WST-1 assay, and the fold changes were compared to control cells. Statistical significance was calculated by Student’s t-test (*p ≤ 0.05; **p ≤ 0.01).