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. 2020 Jun 13;25(12):2746. doi: 10.3390/molecules25122746

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Induction of the caspase cascade after treatment with JIB extract plus cisplatin. OECM-1 cells were treated with JIB extract, cisplatin or a combination of JIB extract and cisplatin for 48 h. Then, the cells were collected, and TUNEL staining was carried out according to the manufacturer’s instructions. Red: PI; green: TUNEL-positive; white arrow: DNA fragments; red arrow: anoikis; yellow arrow: apoptotic bodies. (A) TUNEL staining of OECM-1 cells after drug treatment. (B) The caspase activity of OECM-1 cells treated after drug treatment for 3, 6, 12 and 24 h. *—significant difference between the control group and treatment groups. (p < 0.05).