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. 2020 Jun 17;25(12):2804. doi: 10.3390/molecules25122804

Figure 2.

Figure 2

RL2 induces HMGB1 and ATP release and HSP70 translocation in treated cells. MX-7 and MDA-MB-231 cells were treated with RL2 (0.3 mg/mL) or Doxorubicin (0.1 μg/mL) for 2–48 h. (a,b) Extracellular HMGB1 in RL2- and Dox-treated cells; (c–f) Cellular HMGB1 in RL2-treated samples; western blot analysis of HMGB1 expression in cell lysates, one representative of two independent western blot experiments is shown and (c,e) relative quantification of HMGB1/Tubulin; (g,h) Relative amount of extracellular ATP, measured in cellular medium (RLU, relative luminescent units). (i) Surface-exposed HSP70 revealed by flow cytometry (RL2-treated cells). Median values of three independent experiments are shown ±SE. Statistical differences between control and experimental groups are indicated by * for p < 0.05; ** for p < 0.01, *** for p < 0.001.