Figure 4.
Immunization of C3H/He mice with MX-7 cells treated by recombinant lactaptin analog RL2. (a) Scheme of immunization. Syngeneic mice were s.c. immunized with RL2- or Dox-treated MX-7 cells (7 × 105 cell/animal). Eight days later, the mice were challenged s.c. on the opposite flank with 7 × 105 live MX-7 cells. (b) Dynamics of live and tumor-free mice in groups. (c) Viability of vaccinated mice. (d) Dynamic of tumor growth. The differences in tumor-free mice between groups were calculated using non-parametric statistics, the Pearson′s chi-square test, and these were significant with p < 0.05. Kaplan–Myer survival curves and Log-Rank statistics were calculated using GraphPad Prizm Software (San Diego, CA, USA).