Figure 5.
Ethyl pyruvate (EP) increases the vaccination effect of RL2-treated cells. (a) Scheme of C3H/He mice immunization with MX-7 cells treated by RL2 and EP injections. (b) Dynamic of live and tumor-free mice in groups. Syngeneic mice were s.c. immunized with RL2-treated MX-7 cells (7 × 105 cell/animal) and three days after that mice were i.p. injected with EP (40 mg/kg) according the scheme. Seven days after, the mice were challenged s.c. on the opposite flank with 2.5 × 105 live MX-7 cells. (c) Viability of vaccinated mice. (d) Dynamics of tumor growth. (e) Dynamic of live and tumor-free mice in groups. Syngeneic mice were s.c. immunized with RL2-treated MX-7 cells (5 × 105 cell/animal), EP injected and MX-7 challenged according to the scheme. The differences between groups were calculated using non-parametric statistics, Pearson′s chi-square test, and these were significant with p < 0.05. Kaplan–Myer survival curves and Log-Rank statistics were calculated using GraphPad Prizm Software (San-Diego, CA, USA).