Figure 5.

The effects of caudatin on the CD44^high/CD24^low and aldehyde dehydrogenase (ALDH)-positive cell proportions in a breast cancer cell line. (A) The CD44^high/CD24^low cell population of MDA-MB-231 cells treated with caudatin (100 μM) or DMSO for 24 h was analyzed by flow cytometry. The gating was based on the binding of a control antibody (red cross). (B) MDA-MB-231 cells were treated with caudatin (100 μM) for 24 h and subjected to an ALDEFLUOR assay and FACS analysis. Representative flow cytometric data are shown. The right panel shows the ALDH-positive population treated with the ALDH inhibitor DEAB, and the left panel represents the ALDH-positive population without N,N-diethylaminobenzaldehyde (DEAB) treatment.