Table 1.
List of primers used for real-time PCR.
| Gene | Function | Sequence (5′ → 3′) | Product Length (bp) | Accession Number | Reference |
|---|---|---|---|---|---|
| hilA | Transcriptional regulator of the SPI-1 gene expression | F: CTGTACGGACAGGGCTATCG R: GCAGACTCTCGGATTGAACC |
130 | U25352.1 | [19] |
| prgH | Assembly of base ring structure of T3SS | F: CGCTGCGCAAAATGAAAGAG R: TTACGCGGCTCATCGAAATG |
177 | U21676 | [20] |
| invA | Assembly of needle-like structure of T3SS | F: TCTGGATGGTATGCCCGGTA R: TCATCGCACCGTCAAAGGAA |
140 | M90846.1 | This study |
| sipA | Actin rearrangement | F: GTCATTCGCGTGTGGATTCG R: TTCGGATGAAGCGTTGGTCA |
143 | U40013.1 | This study |
| sipC | Actin rearrangement | F: ACGGGCAGAATAGCGTCAAA R: ATACCCAGACTTTCCGTGGC |
150 | U25631.1 | This study |
| sipD | Translocation of effector proteins | F: ATTCCGCTTCTCCTCATCCG R: ACCGCGATGTTCTGTGGTAG |
107 | U40013.1 | This study |
| sopB | Invasion, generation, and maintenance of SCV | F: GATGGCGGCGAACCCTATAA R: GAAGACTACCAGGCGCACTT |
181 | AF213335.2 | This study |
| sopE2 | Actin rearrangement | F: GAACGCTTCTGAGGGTAGGG R: CGAGCATAGGCCGGATCTTT |
117 | AF200952.1 | This study |
| rpoD | Housekeeping | F: GTGAAATGGGCACTGTTGAACTG R: TTCCAGCAGATAGGTAATGGCTTC |
131 | NC_003197.2 | [21] |