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. 2020 Jun 24;25(12):2907. doi: 10.3390/molecules25122907

Figure 3.

Figure 3

AN07 prevents lipopolysaccharide (LPS)-induced increase in the inducible nitric oxide synthase (iNOS) expression (A), NO production (B), cyclooxygenase-2 (COX2) expression (C), and inhibitor of nuclear factor kappa B-alpha (IκBα) phosphorylation (D) in RAW 264.7 cells. RAW 264.7 cells were pre-treated with AN07 (0.01–1.0 μM) for 1 h and then exposed to LPS (100 ng/mL) for another 24 h. (A,C,D) Upper panels, representative immunoblots. Lower panels, densitometry analyses of the relative ratio of protein/β-actin protein and phosphorylated-IκBα (p-IκBα)/total-IκBα (t-IκBα), which are represented as percentages of the LPS group. (B) Nitrite production was measured using the Griess reagent. The concentration was calculated from a standard curve of reference sodium nitrite. Columns, mean ± S.E.M. from at least three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.