Table 5.
Resistance profile, replicon type, and virulence genes identified in the EC121’s plasmids transferred to E. coli K-12 strains.
| Traits | EC121 | J53 | C600 | ACG04 | ACC09 |
|---|---|---|---|---|---|
| Antimicrobial a | |||||
| ceftazidime | 64 | 0.25 | 0.25 | 8 | 2 |
| ceftriaxone | ≥64 | ≤0.125 | ≤0.125 | >64 | >64 |
| cefepime | 16 | 0.25 | ≤0.125 | 16 | 2 |
| piperacillin/tazobactam | >256 | 2 | 2 | 8 | 2 |
| aztreonam | >32 | ≤0.125 | ≤0.125 | 16 | 4 |
| amikacin | 4 | 2 | 2 | 16 | 2 |
| gentamycin | 16 | 0.5 | 0.5 | 32 | 0.5 |
| trimethoprim/sulfamethoxazole | >128 | ≤0.25 | ≤0.25 | >128 | >128 |
| ciprofloxacin | >64 | ≤0.125 | ≤0.125 | ≤0.125 | ≤0.125 |
| minocycline | 16 | 1 | 1 | 4 | 1 |
| tigecycline | 1 | 0.25 | 0.125 | 0.25 | 0.125 |
| Replicon type b | |||||
| IncHI2 | + | - | - | + | + |
| IncL/M | + | - | - | + | - |
| IncFIB | + | - | - | + | - |
| IncN | + | - | - | + | - |
| Virulence c | |||||
| hlyF | + | - | - | + | - |
| iroN | + | - | - | + | - |
| sitA | + | - | - | + | - |
| Resistance gene | |||||
| bla CTX-M | + | - | - | + | + |
a The antimicrobial resistance phenotype of transconjugant strains was assessed by minimum inhibitory concentration (MIC), performed using the broth microdilution method according to EUCAST guideline, and expressed in µg/mL. Bold values indicate an increased MIC value when comparing strains bearing plasmids with its receptor strain which is devoid of plasmids; b replicons identified in EC121 by PlasmidFinder, and screened by PCR; c virulence genes identified in EC121 genome and screened by PCR; + for the presence of the gene; - for the absence of the gene.