Table 1.

Fragmentation of EcLon by proteolytic enzymes of different specificity

Domain or fragment	Chymotrypsin (present study)			Trypsina		Glutamyl endopeptidaseb
	No. Nuc (+/− Mg2+)	ATP/ADP	ATP/ADP (+ Mg2+)	No. Nuc (+ Mg2+)	ATP/ADP (+ Mg2+)	No. Nucd (+/− Mg2+)	ATP/ADP (+/− Mg2+)
N	1–207	1–207	1–207	1/6–235/239	1/6–235/239	5–240	5–240
A		235–584	235–584
AP			235–784	236/240–784C	236/240–784		241–784
α	491–584
αP				490–784		487–784
P	585–784	585–784	585–784

Nue - nucleotides.

^a,bAccording to literature data (Patterson et al., 2004; Vasilyeva et al., 2002). Some incorrect numbers of amino-acid residues flanking the corresponding tryptic fragments were changed according to the sequencing data presented in the same paper (Patterson et al., 2004).

^cUnstable intermediate fragment.

^didentical results were obtained in the presence of non-hydrolyzable ATP analogs or non-adenosine nucleotides.