Figure 7.

FGFR expression and its influence on OPCs. (a–l) FGFR1 is expressed by OPCs at all the stages analyzed: E16 (a–c), P0 (d–f), P15 (g–i), and P60 (j–l). (m) Histogram showing the results of chemotaxis at P0 and P15 in the presence of SU5402, which reverts the effects of anosmin-1 and FGF2. (n) CNPase⁺ cells at P0 and P15 in cultures treated with 10 µM of SU5402 or the vehicle alone (see Methods). No changes were observed in the presence of SU5402 for P0 but differences existed in P15. (o) FGFR1, R2, and R3 expression by OPCs isolated at E16, P0, P15, and P60. Scale bar represents 7.5 µm for a–c, g–i, and 10 µm for d–f, j–l. The results were analyzed with a Student´s t-test: * P < 0.05, ** P < 0.01, and *** P < 0.001.