Table 6.
Primers and qPCR characteristics of gut microbiota analysis (adapted by Mitsou, et al. [64]).
| Target | Primer | Primer Sequence (5′-3′) | Annealing Temperature | Product Size | Reference Strains | References |
|---|---|---|---|---|---|---|
| Total Bacteria (Universal) | Forward Reverse |
TCCTACGGGAGGCAGCAGT GGACTACCAGGGTATCTAATCCTGTT |
60 °C | 466 bp | Bacteroides fragilis MM44 (ATCC 25285) | [71] |
| Lactobacillus group | Forward Reverse |
AGCAGTAGGGAATCTTCCA CACCGCTACACATGGAG |
58 °C | 341 bp |
Lactobacillus gasseri DSM 20243 |
[72] |
| Bifidobacterium spp. | Forward Reverse |
TCGCGTCYGGTGTGAAAG CCACATCCAGCRTCCAC |
58 °C | 243 bp | Bifidobacterium bifidum DSM 20456 | [72] |
| Bacteroides spp. | Bac303F Bfr-Fmrev |
GAAGGTCCCCCACATTG CGCKACTTGGCTGGTTCAG |
60 °C | 103 bp | Bacteroides fragilis MM44 (ATCC 25285) | [73] |
| Clostridium perfringens group | CPF CPR |
ATGCAAGTCGAGCGATG TATGCGGTATTAATCTCCCTTT |
55 °C | 120 bp |
Clostridium perfringens ATCC 13124 |
[74] |
| Roseburia spp.- Eubacterium rectale | RrecF Rrec630mR |
GCGGTRCGGCAAGTCTGA CCTCCGACACTCTAGTMCGAC |
60 °C | 81 bp |
Roseburia intestinalis DSM 14610 |
[75] |
| Faecalibacterium prausnitzii | FPR-2FFprau645R | GGAGGAAGAAGGTCTTCGG AATTCCGCCTACCTCTGCACT |
60 °C | 248 bp |
Faecalibacterium prausnitzii DSM 17677 |
[76,77,78] |