Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Illustration of target sites and assembly of single guided RNAs (sgRNAs) in pYLCRISPR/Cas9PubiH. (A) The illustration of eight sgRNAs in pYLCRISPR/Cas9. (B) Product length of all sgRNAs in the second round of PCR, M; molecular marker (2000 bp), T1-T8 represents target1-target8 (C) Sequencing confirmation of eight sgRNAs in pYLCRISPR/Cas9PubiH, PAM, protospacer adjacent motif.
