Figure 2.

The CRISPR/Cas9 system induced mutation frequency detection in T₀ generation and sequence alignment of heritable homozygous rice mutants. (A) The mutation efficiency of each sgRNA; (B) types of mutations with the number of mutation events; (C) PCR identification and sequence alignment of wild-type (WT) and four homozygous T₀ mutant lines (i.e., GXU7, GXU19, GXU24, and GXU28). T1–T8 represents Target1-Target8. Red letters are the sgRNA sites; letter in violet color represents inserted nucleotide sequence; the blue letters are protospacer adjacent motif (PAM) sequence; “d” and “i” indicates the deletions and insertion, respectively.