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Grain phenotype and the 2-acetyl-1-pyrroline (2AP) levels of homozygous mutants and wild-type (WT) grains. (A) Grain phenotype of WT and homozygous mutant lines (GXU7-1, GXU19-1, GXU24-1, and GXU27-1). (B) The total ion chromatograms (TIC) of 2AP and TMP (as internal standard) in the T1 homozygous mutant lines and WT. (C) 2AP levels of the mutants and WT. Values are means ± SD, n = 5, p ≤ 0.01, student’s t-test. ** indicates significant difference.
