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. 2020 May 28;9(6):686. doi: 10.3390/plants9060686

Figure 7.

Figure 7

Histochemical observations of laticifers in young leaf midrib sections of T. ventricosa. (a) Presence of polysaccharides within laticifer cells stained using Toluidine Blue. (b) Positive staining of lipids within laticifers using Sudan IV. (c) Negative staining of lipids, cutin, and suberized cell walls of laticifers using Sudan black B. (d) Ferric Trichloride positively stained laticifers a dark-black color. (e) Presence of phenolics within laticifer cells stained using Ferric Chloride. (f) Intense staining of resin acids within laticifers using NADI reagent. (g) Intense staining of neutral lipids within laticifers using Nile Blue. (h) Negative staining of lignin aldehydes within laticifer and cell components stained using Phloroglucinol. (i) Intense blue-black staining of proteins in laticifers. (j) Intense staining of alkaloids within laticifers stained using Wagner’s and Dittmar’s reagent. (k) Positive staining of mucilage and pectin using Ruthenium Red. (l) Presence of acidic substances in laticifers stained using Safranin and Fast Green. Arrows refer to laticifer.