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. 2020 Jun 9;8(6):870. doi: 10.3390/microorganisms8060870

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Deletion of yycH and yycI reduces WalR phosphorylation. (a) Growth and WalR phosphorylation of S. aureus NRS384 walR-FLAG and NRS384 walR-FLAG ΔyycHI as determined by OD₆₀₀ measurement of the cultures and WalR-FLAG detection by quantification of the phosphorylated and non-phosphorylated bands of the Phos-Tag Western blots. (b) Phos-Tag Western blot of S. aureus NRS384 walR-FLAG and NRS384 walR-FLAG ΔyycHI at different time points. In all samples, the phosphorylation of WalR was higher in the wild-type strain than in the yycHI deletion mutant. The experiment was performed three times and the figure shows a representative result.