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Primary HUVECs were exposed to hypoxia (0.9% O2) for up to 24 h. (A) IRE1 (ERN1), (B) XBP1 (total) and (C) XBP1s (spliced) mRNA levels were quantified by quantitative real-time PCR and normalized to 18S and RPLP0 rRNA levels and expressed as a fold change over normoxic samples. Data represent the mean ± SEM of four independent experiments (* p < 0.05 was considered significant).
