Figure 3.

IRE1 knockdown affects HIF1α protein level in hypoxia in HUVECs. (A) IRE1 mRNA levels after IRE1 knockdown. (B) HIF-1α protein levels after IRE1 knockdown in normoxia and hypoxia were evaluated by Western Blotting, normalized to β-actin and total protein levels and related to hypoxia (CTRL). The densitometry analysis is representing two independent experiments (* p < 0.05 was considered significant). (C) HIF1A mRNA levels after IRE1 knockdown. (A) and (C) mRNA levels were quantified by quantitative real-time PCR, normalized to 18S and RPLP0 rRNA levels and expressed as fold changes over normoxic samples. Data represent the mean ± SEM of two independent experiments.