Figure 4.

Low glucose dependence of PlrgA activity during growth in TV medium. The PlrgA-gfp reporter strain in the S. mutans wild type background was grown in TV medium, supplemented by 2 (a), 3 (b), 4 (c), 5 (d), 6 (e), 7 (f) and 11 mM (g) of glucose. Relative gfp expression (green circle) and OD₆₀₀ (white diamond) were monitored during growth on a plate reader (see Materials and Methods for details). The results are representative of three independent experiments.