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In vitro permeability assay in HEC-1A cells. (A) Percentage of E1P47 peptide quantified in each compartment of the Transwell system after treatment with POPC (E1P47) LUVs, PLGA (E1P47) NPs and E1P47, respectively; (B) Lucifer Yellow leakage through HEC-1A monolayer after treatment with E1P47 peptide and without the peptide (blank). The experiment was also carried without cell monolayer (control) to obtain the total leakage of Lucifer Yellow thorough the Transwell membrane inserts.
