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Optimized PCR with specific designed primers for the trnL sequence of P. tamarugo, P. burkartii and P. strombulifera The annealing temperature (Ta) was optimized with DNA samples of Pt6, Pb3, Ps1 and Pr1, by means of a gradient between 50 °C to 65 °C, using the primer pairs PTAM1F-1R, PTAM1F-2R and PSTROM1F-2R. M: is a marker with a molecular weight between 100 to 3000 bp.
