PCR obtained from DNA of six individuals of P. tamarugo, three of P. burkartii, two of P. strombulifera and five of P. reptans, using specified PTAM1F-1R, PTAM1F-2R and PSTROM1F-2R primer pairs at an annealing temperature of 62 °C, 65 °C and 56.5 °C, respectively.