Figure 3.

Significance of the DX2 level on pyrimethamine-mediated suppression of cancer cell viability. DX2- or EV-inducible (ind) A549 cells were treated with doxycycline (Dox) and pyrimethamine (Pyr) at the indicated time to induce the expression of DX2 and to reduce cell viability, respectively. The values on the graph were calculated against cell viability of empty vector (EV)-inducible cell lines at time 0 and presented as a relative cell viability. The experiments were independently repeated three times. Student’s two-tailed t-test was performed for statistical analysis (* p < 0.05, ** p < 0.01). The error bars denote the standard deviation (S.D.), the data shown are the mean ± S.D.