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. 2020 Jun 2;12(6):507. doi: 10.3390/pharmaceutics12060507

Figure 1.

Figure 1

Characterization of fullerene (C60)-polyethyleneimine (PEI)/short hairpin (sh) RNA plasmid nano-polyplexes. (A) Average hydrodynamic diameter and ζ-potential of C60-PEI/shRNA plasmid polyplexes at different N/P ratios. Results are reported as mean ± S.D. for three individual measurements. (B) Agarose gel retardation assay performed for free shRNA plasmid and C60-PEI/shRNA plasmid polyplexes at different N/P ratios (200 ng shRNA plasmid/lane). (C) Viability of VIC exposed for 48 h to different N/P ratios of C60-PEI/shRNA plasmid nano-polyplexes. Data are presented as mean ± S.D. of three experiments made in three replicates (n = 9). * p < 0.05 and *** p < 0.001 versus control cells. (D,E) Uptake of C60-PEI/Cy3-labeled plasmid nano-polyplexes (N/P = 25) by VIC, observed by fluorescence microscopy (D) and by flow cytometry analysis (E). (F) Expression of fluorescent protein in VIC transfected with C60-PEI/pEYFP plasmid at N/P ratio of 15, 20 and 25 or with a commercial transfection reagent, as revealed at 48 h after transfection by fluorescence microscopy (scale bar 200 µm).