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Down-regulation of Runx2 expression using C60-PEI/shRNA-Runx2 plasmids inhibits ALP activity in VIC exposed to HGOM. Quantitative assay at 7, 14 and 21 days (A) and histochemical detection at 14 days (B) of ALP activity (scale bar 100 µm). Data are presented as mean ± S.D. of two experiments made in three replicates (n = 6). * p < 0.05, ** p < 0.01, *** p < 0.001 compared to HGOM; & p < 0.05, && p < 0.01 versus HGOM at day 7.
