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. 2020 Jun 2;9(6):1711. doi: 10.3390/jcm9061711

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cleaved caspase 3 immunostaining: (A) Rat cochlear slices were subjected to cleaved caspase 3 immunostaining (red) to determine apoptosis. Cell nuclei were stained with DAPI (blue). Cochleae harvested from rats that received BM-MSCs, PBS injected, or control group showed no or sparse staining whereas those from the positive group showed intense staining (red color). Blue color shows DAPI staining. (B) Mean signal intensity for cleaved caspase 3 was calculated using Image J software. Data are expressed as mean values ± standard deviation (SD). WC: whole cochlea; SGNs: Spiral Ganglion Neurons; HCs: Hair Cells; SL: Spiral Ligament.