Figure 3.

Sensitivity of the ubc7/13/14 seedlings to different concentrations of stress reagents. The WT and ubc7/13/14 mutant seeds were sterilized, stored at 4 °C, and then plated onto the normal or treatment plates containing the stress reagents at the indicated concentrations. In each plate, seven WT and seven mutant seeds were planted side by side. The primary root length was measured using ImageJ on the 14th day after seed plating. For easy comparisons among treatments, the root length of WT seedlings in the normal plates was set to 100%. Each treatment had five replicate plates. The significance of difference between the mutant and WT was analyzed using the Student’s t-test (error bar = SE; *: p < 0.05; **: p < 0.01). (A) 120 mM NaCl, (B) 3% mannitol, (C) 0.15 μM PBI425 (an ABA analog), and (D) 0.03 μM paraquat.