Figure 4.

Sensitivity of single to triple ubc7/13/14 mutants to salt stress. Seedlings of WT and mutant seedling growing on the medium plates containing 150 mM NaCl. Arabidopsis WT and mutant seeds were sterilized, stored at 4 °C, and then planted on the salt-containing plates (each plate having 50 seeds). The plates were placed in a tissue culture chamber. (A) Representative images of the plates photographed on the 14th day after seed plating. The single, double, and triple mutants are indicated as ubc7, ubc13, ubc14, ubc7/13, ubc7/14, ubc13/14, and ubc7/13/14. (B) The percentage of bleached seedlings. Each plant line had five plates with each plate having 50 seeds. Seedlings were surveyed on the 14th day after seed plating. One-way ANOVA and post-hoc Tukey test were used to analyze the data, and significant differences are indicated by different letters (uppercase at p < 0.01 level).