Figure 5.

Complementation of the ubc7/13/14 mutant by UBC7. A genomic UBC7 fragment including the promoter region was introduced into the ubc7/13/14 mutant. The seedlings of the WT control, the ubc7/13/14 mutant, and four complementation (T2) lines were grown on medium plates containing 150 mM NaCl, and a survey of bleached seedlings was taken on the 14th day after seed plating. Each line had five plates with each having 50 seeds. One-way ANOVA and post-hoc Tukey test were used to analyze the data, and significant differences are indicated by different letters (uppercase at p < 0.01 level).