Figure 7.

Sensitivity of the ubc7/13/14 seedlings to tunicamycin. (A,B) The WT and ubc7/13/14 mutant seeds were sterilized, stored at 4 °C, and then plated onto the plates with different concentrations of tunicamycin. In each plate, seven WT and seven mutant seeds were planted side by side. There were four replicate plates for each treatment and they were placed vertically in a tissue culture chamber. (A) Representative images of WT and mutant seedlings (14-day-old) on control, 25, 50 and 75 ng/mL tunicamycin plates. (B) Relative root length. Images were taken on the 14th day after seed plating and the average root length of the seven seedlings from each plate obtained (root lengths measured using ImageJ). The root length of WT seedlings in the normal plates was set to 100%. (C) Survey of severely inhibited seedlings (dark brown and small) was taken on the 14th day after seed plating. For each concentration, each plant line had four replicate plates with each plate having 50 seeds. Plates were placed horizontally in a tissue culture chamber. The significance of difference between the mutant and WT was analyzed using the Student’s t-test (error bar = SE; **: p < 0.01).