Table 5.
Caspase-3/7 activities after treatment with NZ surf clam extracts.
| Cell line/Extracts | Oven dried (OD) treatment |
Freeze-dried (FD) treatment |
||
|---|---|---|---|---|
| Caspase-3/7 activity (%) | Caspase-3/7 activity (%) | |||
| A549 | C | 100 | C | 100NS |
| DS | DScd 400 μg/ml | 165 | DSea 400 μg/ml | 228∗∗∗ |
| DScd 600 μg/ml | 216 | DSea 600 μg/ml | 301∗∗∗∗ | |
| SS | SSea 400 μg/ml | 257 | SSea 400 μg/ml | 494∗∗∗∗ |
| SSea 600 μg/ml | 282 | SSea 600 μg/ml | 591∗∗∗∗ | |
| TT |
TTpe 400 μg/ml | 345∗ | TTea 400 μg/ml | 338 |
| TTpe 600 μg/ml | 378 | TTea 600 μg/ml | 401∗∗∗ | |
| Hep G2 | C | 100 | C | 100 NS |
| DS | DSet 400 μg/ml | 474 | DSea 400 μg/ml | 750∗∗∗∗ |
| DSet 600 μg/ml | 445 | DSea 600 μg/ml | 757∗∗∗∗ | |
| SS | SSpe 400 μg/ml | 485 | SSea 400 μg/ml | 977∗∗∗∗ |
| SSpe 600 μg/ml | 490 | SSea 600 μg/ml | 979∗∗∗∗ | |
| TT |
TTpe 400 μg/ml | 384 | TTea 400 μg/ml | 551∗∗∗∗ |
| TTpe 600 μg/ml | 455 | TTea 600 μg/ml | 685∗∗∗∗ | |
| MCF-7 | C | 100 | C | 100 NS |
| DS | DSea 400 μg/ml | 653∗∗∗∗ | DSpe 400 μg/ml | 472 |
| DSea 600 μg/ml | 903∗∗∗∗ | DSpe 600 μg/ml | 801 | |
| SS | SSpe 400 μg/ml | 679 | SScd 400 μg/ml | 758∗∗∗∗ |
| SSpe 600 μg/ml | 659 | SScd 600 μg/ml | 895∗∗∗∗ | |
| TT |
TTpe 400 μg/ml | 590 | TTpe 400 μg/ml | 913∗∗∗∗ |
| TTpe 600 μg/ml | 447 | TTpe 600 μg/ml | 789∗∗∗∗ | |
| MIA PaCa-2 | C | 100 | C | 100 NS |
| DS | DSea 400 μg/ml | 236 | DSpe 400 μg/ml | 395∗∗∗∗ |
| DSea 600 μg/ml | 236 | DSpe 600 μg/ml | 520∗∗∗∗ | |
| SS | SSet 400 μg/ml | 305 | SSpe 400 μg/ml | 474∗∗∗∗ |
| SSet 600 μg/ml | 302 | SSpe 600 μg/ml | 725∗∗∗∗ | |
| TT |
TTpe 400 μg/ml | 225 | TTpe 400 μg/ml | 276∗∗∗ |
| TTpe 600 μg/ml | 180 | TTpe 600 μg/ml | 284∗∗∗∗ | |
| PC-3 | C | 100 | C | 100 NS |
| DS | DSea 400 μg/ml | 280∗∗∗ | DSpe 400 μg/ml | 246 |
| DSea 600 μg/ml | 530∗∗∗∗ | DSpe 600 μg/ml | 338 | |
| SS | SSet 400 μg/ml | 374∗∗∗ | SSpe 400 μg/ml | 351 |
| SSet 600 μg/ml | 454 | SSpe 600 μg/ml | 667∗∗∗∗ | |
| TT |
TTet 400 μg/ml | 248 | TTpe 400 μg/ml | 475∗∗∗∗ |
| TTet 600 μg/ml | 301 | TTpe 600 μg/ml | 395∗∗∗∗ | |
| SiHa | C | 100 | C | 100 NS |
| DS | DSpe 400 μg/ml | 261 | DSea 400 μg/ml | 439∗∗∗∗ |
| DSpe 600 μg/ml | 373 | DSea 600 μg/ml | 539∗∗∗∗ | |
| SS | SSpe 400 μg/ml | 379 | SSea 400 μg/ml | 550∗∗∗∗ |
| SSpe 600 μg/ml | 683 | SSea 600 μg/ml | 1066∗∗∗∗ | |
| TT |
TTpe 400μg/ml | 244 | TTpe 400μg/ml | 434∗∗∗∗ |
| TTpe 600 μg/ml | 294 | TTpe 600 μg/ml | 575∗∗∗∗ | |
| WiDr | C | 100 | C | 100 NS |
| DS | DSea 400 μg/ml | 865∗∗∗∗ | DSpe 400 μg/ml | 480 |
| DSea 600 μg/ml | 1268 | DSpe 600 μg/ml | 1377∗∗∗∗ | |
| SS | SSet 400 μg/ml | 1436∗∗∗∗ | SSpe 400 μg/ml | 1014 |
| SSet 600 μg/ml | 973 | SSpe 600 μg/ml | 1896∗∗∗∗ | |
| TT | TTet 400 μg/ml | 280 | TTpe 400 μg/ml | 1056∗∗∗∗ |
| TTet 600 μg/ml | 589 | TTpe 600 μg/ml | 671∗∗∗∗ | |
Caspase-3/7 activities were evaluated by Apo-ONE Homogeneous Caspase-3/7 Assay kit. The Caspase-3/7 activity of each group was indicated by their rate fluorescence (RFU). Experiments were carried out twice, in triplicate. Data is presented as mean ± SE. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.005; ∗∗∗∗p < 0.0005; NS = not significant (Student's t-test). C: control.