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Cytotoxicity and hemolytic assay of hepcidin-20 and -25. (A) L8824 cells were incubated with varying concentrations (0 to 100 μM) of peptides for 24 h at 28 °C. Cell viability was assessed using a CCK-8 kit. (B) Hemolytic activities of peptides (0 to 100 μM) were detected with 2% grass carp erythrocytes for 1 h at 28 °C. 2% Triton X-100 was used as a positive control.
