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. 2020 May 28;10(6):825. doi: 10.3390/biom10060825

Figure 6.

Figure 6

Therapeutic and preventive effects of hepcidin-20 and 25 in L8824-infected bacteria. (A–D) Bacterial suspension in M199 was incubated with L8824 cells for 3 h and then treated with PBS (B), hepcidin-20 (C), or -25 (D) (1 × MBC 16 μM) at 28 °C for 24 h. Cell density was determined using phase-contrast microscopy and crystal violet staining. Uninfected cells (A) were used as blank control. (E) After completing the above processing, the resulting cell culture medium was plated on agar plates for colony counts. (F) L8824 cells were treated with PBS, hepcidin-20, or -25 for 3 h, and then bacterial suspension in M199 was incubated with cells for 24 h. Subsequently, cell density was determined using phase-contrast microscopy and crystal violet staining. (G) The CFU assay was employed to investigate the antibacterial activity of hepcidin-20 and -25 in the M199 medium. Image shown were representative of three independent experiments, * p < 0.05; ** p < 0.01.