Figure 7.

The effects of hepcidin-20 and 25 on iron homeostasis. HEK293T cells were seeded in 6-well plates 24 h before transfection with Fpn-eGFP. 24 h after transfection, cycloheximide (75 μg/mL) was added for 3 h, and then cells were incubated with PBS (A), hepcidin-20 (1 μM) (B) or hepcidin-25 (1 μM) (C) for at least 24 h. (D) L8824 cells were seed in 12-well plates for 24 h. Next, cells were stimulated with hepcidin-25 or hepcidin-20, and the LIP content was detected by fluorometric assay.