Table 1.

Brief summary of all the nucleic acid vectors.

SN	Nucleic Acid	Gene/Target	Vector	Disease/Condition	Route of Delivery	Animal/Cell Line Used	Ref.
1	mRNA	EPO	LNPs	-	IV	C57BL/6 mice	[35]
2	mRNA	Luciferase	LNPs	-	IP, IM, SC, IV, ID and ITr	BALB/c mice	[34]
3	mRNA	FXN	LNPs	Friedreich’s ataxia	ICV	BALB/c mice	[36]
4	mRNA	Luciferase and hEPO	LNPs	-	IV	CD-1 mice, rats, cynomolgus monkeys	[37]
5	mRNA	pRM and E	LNPs	Zika	IM	AG129 mice	[41]
6	siRNA and mRNA	Factor VII and luciferase	LNPs	-	IV	C57BL/6 mice	[53]
7	siRNA	PCSK9	LNPs	Elevated LDL-Cholesterol	IV	C57BL/6 mice and monkeys	[56]
8	mRNA	PCSK9	LNPs	Elevated LDL-Cholesterol	IV	BALB/c mice	[72]
9	G3139-GAP	Bcl-2	LNPs	Lung cancer	IV	BALB/c mice	[74]
10	mRNA	Luciferase	hPBAEs NPs	-	Inhalation	C57BL/6 mice	[88]
11	mRNA	EPO	PACE NPs	-	IV	BALB/c mice	[89]
12	siRNA	CIITA	PACE NPs	Tissue transplantation	Incubation	SCID/beige mice	[91]
13	siRNA	Nogo-B	PACE NPs	Hepatic fibrosis and alcoholic liver disease	Spleen	C57BL/6 mice	[92]
14	ASO	Human telomerase	Chitosan-coated PLGA NPs	Lung cancer	Cellular uptake	A549 cancer cell line	[97]
15	ASO	miR-21	PACE NPs	Glioblastoma	CED	Fischer 344 rats	[103]
16	siRNA	PLK-1	EGF antibody Anti-HB -LNPs	Triple negative breast cancer	IV	BALB/c mice	[110]
17	siRNA	ApoB	PBAVE NPs	-	IV	ICR mice	[132]
18	ZFN mRNA	SFTB	Chitosan-coated PLGA NPs	Lung disease	ITh	SP-B transgenic mice	[140]
19	ZFN mRNA	TTR and PCSK9	LNPs	Elevated LDL-Cholesterol and amyloidosis	IV	CD-1 mice	[141]
20	Cas9 and sgRNA	EGFP	PEI coated DNA nanoclew	Osteosarcoma	IT	Nude mice	[144]
21	Cas9 and sgRNA	PCSK 9 and HBV	LLNs	Elevated LDL-Cholesterol and Hepatitis B	IV	C57BL/6 mice	[142]

Abbreviations: EPO, erythropoietin; LNPs, lipid nanoparticles; FXN, frataxin; FVII, protein Factor VII; PCSK9, proprotein convertase subtilisin/kexin type 9; bcl-2, B-cell lymphoma 2; prM, pre-membrane protein; E, envelope protein; CIITA, class II transactivator; Nogo-B, Nogo-B gene, a part of Nogo/Reticulon-4B family; hPBAEs, hyper branched poly-β-amino esters; PACE, poly(amino-co-ester); PLGA, poly(lactic-co-glycolic acid); miR-21, microRNA-21; PLK-1, polo-kinase 1; ApoB, apolipoprotein; PBAVE; poly(butyl amino vinyl ether) polymer; PEI, polyethylenimine; TTR, transthyretin; EGFP, epidermal growth factor protein; SFTB, surfactant B gene encoding for surfactant B protein; sgRNA, single guide RNA; HBV, hepatitis B virus; IV, intravenous; IP, intraperitoneal; IM, intramuscular; SC, subcutaneous; ID, intradermal; ITr, intratracheal; ICV, intracereboventicular; IT, intratumoral; ITh, intrathecal; CED, convection enhanced delivery.