Table 2.
Quantitative analysis of maackiain and other isoflavonoids from UHPLC in S. flavescens callus and explant (n = 3 biological replicates).
| Maackiain | Kurari-Methoxy | Kush F | Kurari | Kush E | |
|---|---|---|---|---|---|
| Root * | 145.9a | 468.6b | 1621.4d | 299.1a | 0 |
| Root (in vitro) ** | 123.3a | 69.2a | 933.1c | 781.3b | 0 |
| L58 | 1580.6d | 0 | 229.1b | 0 | 0 |
| R76 | 653.7c | 0 | 0 | 0 | 0 |
| S18 | 428.3b | 0 | 0 | 0 | 0 |
| S55 | 592.4c | 0 | 182.6b | 0 | 0 |
| S88 | 395.9b | 0 | 14.8a | 0 | 0 |
* Indicates that the materials came from field-grown S. flavescens root; ** indicates that the materials came from in vitro S. flavescens root (μg/g dry weight). Different letters indicate significant difference at the 5% level by Duncan’s multiple range test. UHPLC: Ultra-High Performance Liquid Chromatography. Kush F: Kushenol F. Kurari: Kurarinone. Kush E: Kushenol E.