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. 2020 May 30;8(6):825. doi: 10.3390/microorganisms8060825

Figure 1.

Figure 1

Light micrographs and karyotypes of Culicoides nubeculosus cell lines CNE/LULS44 and CNE/LULS47. (a) CNE/LULS44 cells at passage 15, 24 months after initiation, live, phase contrast, scale bar = 100 µm; (b) CNE/LULS47 cells at passage 3, 14 months after initiation, live, phase contrast, scale bar = 100 µm; (c) CNE/LULS44 cells at passage 13, Giemsa-stained cytocentrifuge smear, scale bar = 10 µm; (d) CNE/LULS47 cells at passage 5, Giemsa-stained cytocentrifuge smear, scale bar = 10 µm; (e) chromosome preparation from CNE/LULS47 cells at passage 6, showing three chromosome spreads (circled) with diploid number 2n = 6, Giemsa stain, scale bar = 10 µm; (f) distribution of chromosome numbers in 110 metaphase chromosome spreads each of CNE/LULS44 cells at passage 13 (grey bars) and CNE/LULS47 cells at passage 6 (white bars).