Figure 2.

Induction of brain-derived neurotrophic factor (BDNF) in SH-SY5Y cells by ginsenoside Rf (g-Rf). (A,C) Cells were incubated with 10 μM g-Rf for the indicated time periods. (B,D) Cells were incubated with the indicated concentrations of g-Rf for 8 h (B) or 24 h (D). Total RNA and whole-cell lysates were prepared and subjected to real-time PCR (A,B) and immunoblot analysis (C,D). Data are presented as mean ± SE (n = 3 or 4). *p < 0.05, **p < 0.01 compared with untreated group.