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Induction of BDNF in SH-SY5Y cells by taurine. (A,C) Cells were incubated with 25 μM taurine for the indicated time periods. (B,D) Cells were incubated with the indicated concentrations of taurine for 8 h (B) or 24 h (D). Total RNA and whole-cell lysates were prepared and subjected to real-time PCR (A,B) and immunoblot analysis (C,D). Data are presented as mean ± SE (n = 3 or 4). *p < 0.05, **p < 0.01 compared with untreated group.
