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. 2020 Jun 17;12(6):561. doi: 10.3390/pharmaceutics12060561

Figure 7.

Figure 7

Confocal images of SH-SY5Y and Vero cells exposed to magnetite/silver-pDMAEMA-PEA-BUFII-RhodB nanobioconjugates for 0.5 and 4 h. (A) The first two channels correspond to nuclei (blue) and endosomes (green) labeled with Hoechst 33342 and Lysotracker green DND-26, respectively. The third channel corresponds to nanobioconjugates labeled with rhodamine-B (red) and the fourth to the DIC field that shows cell morphology and integrity. Finally, the merge of all channels’ highlights nanoparticle cellular internalization (yellow areas correspond to colocalization between red and green channels). (B, C) SH-SY5Y and Vero cells exposed to magnetite/silver-pDMAEMA-PEA-BUFII-RhodB nanobioconjugates for 0.5 h and 4 h. The 3D reconstructions show the distribution of the nanobioconjugates throughout the cells’ cytoplasm. The white arrows point to endosomal escape areas (red areas), while the yellow ones showed colocalization of the nanobioconjugates and endosomes where no endosomal escape was achieved (yellow areas).