Table 1.

Selection of 6 regions with base substitutions; variants are coded as initial base/amino acid-position after startcodon-base substitution; reference sequence for all analyses: MT159713.

Selected Region	1	2	3	4	5	6
Variant Sequence	MT163718	MT163718	MT163718	MT163718	LR757995	MT163718
Total number sequences with variation	26	6	6	9	2	26
Gene	orf 1 ab gene, nsp4 peptide	orf 1 ab gene, nsp13 peptide	orf 1 ab gene, nsp13 peptide	orf 1 ab gene, nsp14 peptide	NS8 gene	NS8 gene
Function	contains transmembrane domain	helicase and others	helicase and others	exonuclease	unknown	unknown
Variant Nucleotide	C8517T	C17483T	A17593G	C17795T	C32T	T251C
Variant Amino Acid	S2839S	P5828L	Y5865C	L5932L	T11I	L84S
Consequence	silent mutation	effective variant	effective variant	silent mutation	effective variant	effective variant
Substitution in antigenic region	not relevant	no	no	not relevant	yes	yes