Source of Variations |
d.f |
Phytochemicals and Antioxidant Activity |
C-glycosyl flavone (µg/g) |
DPPH activity (%) |
FRAP Activity (Fe (II)/g) |
Total Phenolic Content (mg quercetin/g DW) |
Total Flavonoid Content (mg GAE/g DW) |
Shaftoside |
Isoorientin |
Orientin |
Isovitexin |
Vitexin |
Block |
4 |
1.05 |
616.55 |
0.12 |
0.94 |
0.84 |
1.83 |
0.22 |
0.72 |
0.18 |
Harvesting age (HA) |
2 |
49.85 ** |
10034.29 ** |
95.17 ** |
34.73 ** |
7.03 |
0.82 |
0.03 |
0.28 |
0.17 |
Harvesting frequency (HF) |
2 |
97.23 ** |
25343.55 ** |
75.63 ** |
22.47 ** |
77.16 * |
14.39 * |
1.71 |
0.94 |
0.59 |
Interaction (HA × HF) |
4 |
1.38 |
1301.28 * |
3.62 ** |
0.04 |
40.53 |
0.24 |
0.13 |
0.48 |
0.34 |
Error |
32 |
0.59 |
371.59 |
0.18 |
0.61 |
17.42 |
1.59 |
0.09 |
0.56 |
0.20 |
Total |
44 |
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Contrast |
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Linear (HA) |
1 |
33.20 ** |
6621.36 * |
54.57 * |
22.68 * |
2.55 |
0.355 |
0.01 |
0.25 |
0.11 |
Quadratic (HA) |
1 |
0.03 |
67.14 |
8.83 ** |
0.50 |
2.16 |
0.00 |
0 |
0.24 |
0.01 |
Linear (HF) |
1 |
64.77 ** |
16759.20 ** |
50.04 ** |
14.91 ** |
51.10 * |
11.32 * |
1.13 * |
0.33 |
0.11 |
Quadratic (HF) |
1 |
0.05 |
137.13 |
0.33 * |
0.04 |
0.3 |
2.78 |
0.02 |
0.3 |
0.01 |
* and ** are significantly difference at p < 0.05 and p < 0.01 respectively. |