Figure 4.

Binding of RBP reporters to B. anthracis cells varies between RBP of different phages but yields strongest signals during logarithmic growth phase of host cells. Growth experiments were started from germinating B. anthracis Sterne spores and the growth phase was derived from recordings in optical cell densities at 600 nm (OD₆₀₀). Binding strengths of reporters RBP_AP50, RBP_λ03Δ1-120 or RBP_Wip are indicated by colored sections of the growth curves: grey = no binding, light red = weak RBP binding, i.e., either only sporadic binding and/or binding to only a few areas of the cell surface. red: distinct RBP binding (easily recognizable binding to the majority of all cells). dark red = very strong RBP binding ((almost) all cells exhibit very intense fluorescence signals).