Figure 6.

Binding of RBP reporters to encapsulated cells of B. anthracis. Fresh cultures of B. anthracis strain Ames inoculated from overnight cultures were grown for 4 h under capsule-inducing conditions and then inactivated by peracetic acid treatment. Cells were incubated with RBP reporters (RBP_AP50, RBP_λ03Δ1-120 or RBP_Wip) or no RBP (control) and subjected to fluorescence microscopy in the presence of negative-staining ink. Representative micrographs were recorded as merged light and fluorescence channels. Scale bars: 10 µm.